Product Name

Bovine Immunoglobulin G (IgG) ELISA Kit

Catalogue #: RK00471

Size: 96T

Sample: 100ul

Instrument: microplate reader

Detection: Colorimetric

Additional Information

Cross-reactions with bovine

Product Overview

The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in serum, plasma, cell culture supernatants, and urine.

Intended Use

This Bovine Immunoglobulin G (IgG) ELISA Kit is intended for laboratory research use only and is not intended for use in diagnostic or therapeutic procedures. The stop solution changes colour from blue to yellow and the intensity of the colour is measured at 450 nm using a spectrophotometer.

To measure bovine immunoglobulin G (IgG) concentration in the sample, this bovine immunoglobulin G (IgG) ELISA kit includes a set of calibration standards. Calibration standards are run at the same time as the samples and allow the operator to produce a standard curve of optical density versus bovine immunoglobulin G (IgG) concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve.

Storage Instructions

Store unopened kit at 2-8°C for 1 week. If it is more than a week, store the kit components according to the instructions. Do not use the kit after the expiration date.

Sensitivity: 0.55ug/mL

Standard curve range: 1.23-100 ug/ml

Components

• Microtiter plate
• enzyme conjugate
• standards
• Substrate A
• Substrate B
• stop solution
• wash solution
• equilibrium solution

Safety Notes

1. This kit contains materials with small amounts of sodium azide. Sodium azide reacts with lead and copper plumbing to form explosive metal azides. Upon disposal, flush drains with a large volume of water to prevent azide buildup. Avoid ingestion and contact with eyes, skin or mucous membranes. In case of contact, rinse the affected area with plenty of water. Observe all federal, state, and local regulations for disposal.
2. All blood components and biological materials must be handled as potentially hazardous. Follow universal precautions established by the Centers for Disease Control and Prevention and the Occupational Safety and Health Administration when handling and disposing of infectious agents.