Mouse IgM ELISA Kit

Properties

Species reactivity: mouse

Technique(s)

ELISA: adequate

Test range

  • cv between trials: <10%
  • intra-assay cv: <12%

Sent in: wet ice

Storage temperature: −20°C

General description

Immunoglobulin M (IgM) is a highly conserved antibody in vertebrates and shows early expression during the immune response. Peritoneal B cells secrete IgM and it exists as a pentamer. IgM plays a key role in the uptake of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and an increased risk of infections. Mouse IgM Elisa Kit shows polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating the clearance of tissue-based molecules. The antibody pair provided in this kit recognizes mouse IgM.

Application

  • For research use only. It should not be used in diagnostic procedures.
  • See attached general ELISA KIT procedure (sandwich, competitive and indirect ELISA)

Mouse IgM ELISA Kit (A1572) | Antibodies.com

Other notes

On occasion, small volumes of Mouse IgM Elisa Kit vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly spin the vial in a tabletop centrifuge to dislodge any liquid in the vial cap. Certain products may require dry ice shipping and an additional dry ice fee may apply.

Search terms for the purchase of Mouse IgM

MBS564075 is a ready-to-use microwell, strip, or full plate ELISA (Enzyme-Linked Immunosorbent Assay) Kit for testing the presence of Mouse IgM ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls generate a theoretical detection range of the kit in biological research samples containing mouse IgM.

The MBS564075 ELISA analytical biochemical technique is based on mouse IgM antibody-mouse IgM antigen interactions (immunosorbent) and an HRP colourimetric detection system to detect mouse IgM antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant mouse IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, and secretions. Quality control assays that evaluated reproducibility identified intra-assay CV (%) and inter-assay CV (%).

Product related information for Mouse IgM ELISA Kit

Intended Uses: The IgM Test Kit is a highly sensitive two-site enzyme-linked immunoassay (ELISA) for measuring IgM in mouse biological samples. If the ELISA is to be used outside of its intended use, the user may need to optimize it for that use.

Assay Principle: The principle of the double antibody sandwich ELISA is depicted in Figure 1. In this assay, IgM present in samples reacts with anti-IgM antibodies, which have been adsorbed on the surface of microtiter wells of polystyrene. After removing unbound proteins by washing, horseradish peroxidase (HRP)-conjugated anti-IgM antibodies are added. These enzyme-labelled antibodies form complexes with previously bound IgM.

After another wash step, the immunosorbent-bound enzyme is analyzed by the addition of a chromogenic substrate, 3,3′,5,5′-tetramethylbenzidine (TMB). The amount of bound enzyme varies directly with the concentration of IgM in the tested sample; therefore, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The amount of IgM in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.

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