Product name
Transferrin (TF), ELISA kit
Full product name
Bovine Transferrin ELISA Kit
Product gene name
TF Elisa kit
For research use only
For research use only. It should not be used in diagnostic procedures.
Species reactivity: Bovine
Specificity
No significant cross-reactivity or interference between bovine TF and analogues was observed.
Samples
Serum, plasma, tissue homogenates, feces and urine
Test type: sandwich
Detection range: 31.2mg/dl – 1000mg/dl.
Sensitivity: 5.0mg/dl.
Intended uses
This Quantitative Sandwich ELISA Kit is for in vitro research use only, not for therapeutic, home, or drug applications! It is intended to determine the concentrations of TF in serum, plasma and other bovine body fluids. Using the purified bovine TF antibody to coat the wells of the Microelisa strip plate to make the solid phase antibody, then add TF and the TF antibody that has been HRP-labeled to the wells, then the reagents are converted into a complex of antibody-antigen-antibody-enzyme, after washing thoroughly, add TMB substrate solution, TMB substrate turns blue under catalyzed by HRP enzyme, the reaction is finished by adding sulfuric acid solution and changing colour is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay precision
The intra-assay CV (%) is less than 15%.
Inter-assay precision
The inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degrees C
Product note
Information selected from the online data sheet is extracted from bioinformatics databases, sometimes resulting in the ambiguous or non-relevant product information. It is the customer’s responsibility to review, verify and evaluate the information to ensure it matches their requirements prior to purchasing the kit. Our ELISA Kit assays are dynamic research tools and may be updated and improved from time to time.
If the format of this assay is important to you, please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current trial details. We cannot guarantee that the sample manual posted online is the most up-to-date manual, it is intended to serve as an example only. Consult the instructions for use provided with the assay kit for precise details.
Other notes
Small volumes of bovine transferrin Elisa kit vials may occasionally become trapped in the product vial seal during shipping and storage. If necessary, briefly spin the vial in a tabletop centrifuge to dislodge any liquid in the vial cap. Certain products may require dry ice shipping and an additional dry ice fee may apply.
Search terms for the purchase of TF
MBS025326 is a ready-to-use microwell strip plate ELISA (Enzyme-Linked Immunosorbent Assay) Kit for testing the presence of Transferrin (TF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls generate a theoretical detection range of the kit in biological research samples containing TF.
The analytical biochemical ELISA technique of the MBS025326 kit is based on TF antibody-TF antigen (immunosorbent) interactions and an HRP colourimetric detection system to detect TF antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant TF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, and secretions. Quality control assays that evaluated reproducibility identified intra-assay CV (%) and inter-assay CV (%).
Product related information for Elisa kit
Background: This quantitative sandwich ELISA kit is for in vitro research use only! It is intended to determine the concentrations of TF in serum, plasma and other bovine body fluids. Using the purified bovine TF antibody to coat the wells of the Microelisa strip plate to make the solid phase antibody, then add TF and the TF antibody that has been HRP-labeled to the wells, then the reagents are converted into a complex of antibody-antigen-antibody-enzyme, after washing thoroughly, add TMB substrate solution, TMB substrate turns blue under catalyzed by HRP enzyme, the reaction is finished by adding sulfuric acid solution and changing colour is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Precautions
All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic, or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe the intended use will not meet our acceptable guidelines.
Disclaimer
While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource shall not be responsible for any omissions or errors contained in this document. MyBioSource reserves the right to make changes to this datasheet at any time without notice.
